A Simple Key For principle of hplc instrument Unveiled
The most typical RP stationary phases are based upon a silica assistance, that's surface area-modified by bonding RMe2SiCl, wherever R is a straight chain alkyl team like C18H37 or C8H17.By lowering the pH with the solvent in the cation exchange column, By way of example, a lot more hydrogen ions can be obtained to compete for positions around the